UPLC-QTOF/MS-Based Lipidomic Profiling of Liver Qi-Stagnation and Spleen-Deficiency Syndrome in Patients with Hyperlipidemia
Table 4
Identification results of varying ions and their change trend of hyperlipidemia with syndrome of SLQSD and hyperlipidemia with the syndrome of non-SLQSD.
NO
Retention Time
Mass
Group 3Content
Group 4 Content
Factor of change
VIP value
Change Trend
Identified potential Biomarker
Lipid Class
Ion
1
5.53
544.3342
9.3314±3.2342
16.2656±4.2760
0.6
7.2994
↓
LysoPC(20:4)
Lysophospholipid
M+H
2
12.28
784.5734
14.3592±3.3714
7.7689±1.4901
1.8
10.0211
↑
PC(18:3/18:0)
Glycerophospholipid
M+H
3
12.34
787.5918
11.4418±1.1513
3.6700±1.7245
3.1
6.0391
↑
TG(14:0/18:3/15:0)
Glycerolipids
M+H
4
12.64
806.5652
5.7728±1.0044
2.2932±0.7673
2.5
6.1186
↑
LacCer(d18:1/12:0)
Sphingomyelin
M+H
5
15.81
782.5518
246.9174±38.6396
196.1073±11.3457
1.3
63.2972
↑
PC(20:3/16:1)
Glycerophospholipid
M+H
6
16.48
784.5824
38.2776±5.4524
8.4079±0.3322
4.6
16.5178
↑
PC(18:3/18:0)
Glycerophospholipid
M+H
7
16.69
759.5692
57.5260±7.8236
40.4171±4.7738
1.4
12.2461
↑
SM(d18:1/20:0)
Sphingolipid
M+H
8
16.86
810.5915
19.9225±0.8748
274.9301±17.5735
0.1
2.44919
↓
PC(22:4/16:0)
Glycerophospholipid
M+H
9
18.30
758.5560
135.1895±8.1870
256.3050±30.1737
0.5
36.2027
↓
PE(22:2/15:0)
Glycerophospholipid
M+H
10
8.50
508.3418
43.0992±4.4230
79.1179±18.2387
0.5
3.03607
↓
LysoPE(20:0/0:0)
Lysophospholipid
M-H
11
14.32
876.5754
7.7353±2.4394
4.7881±0.1727
1.6
2.02951
↑
PC(22:6/22:6)
Glycerophospholipid
M-H
12
14.38
351.2478
15.6962±5.1028
5.7276±0.27980
2.8
2.29853
↑
DG(20:2/22:0/0:0)
Glycerolipids
M-2H
13
15.05
800.5540
22.9620±3.8468
15.0764±4.0387
1.5
2.17423
↑
PE(22:5/20:1)
Glycerophospholipid
M-H20-H
14
15.3
826.6140
17.8481±1.8121
4.9814±1.1049
3.6
1.14469
↑
PC(22:6/18:3)
Glycerophospholipid
M-H
15
15.94
852.6908
17.8481±1.8121
4.9814±1.1049
3.6
2.44998
↑
PE(24:0/20:3)
Glycerophospholipid
M-H
16
16.00
841.5830
11.7030±2.4241
6.2152±1.0354
1.9
1.77427
↑
PI(16:0/20:3)
Phosphatidylinositol
M-H20-H
17
16.25
854.6047
101.6059±23.4842
11.3739±0.8854
8.9
2.41923
↑
PC(22:4/20:5)
Glycerophospholipid
M-H
18
16.45
857.5742
23.9200±0.5182
6.4069±1.1494
3.7
1.47236
↑
PI(16:0/20:4)
Phosphatidylinositol
M-H
Group 3: hyperlipidemia with syndrome of SLQSD and Group 4: hyperlipidemia with the syndrome of non-SLQSD. ↑ indicated the concentrations compared to the other group are increasing, and ↓indicated the concentrations compared to the other group are reducing. P<0.05 and P<0.01. PE: phosphatidylethanolamine; PC: phosphatidylcholine; TG: triglyceride; DG: diglyceride; PI: phosphatidylinositol; SM: sphingomyelin; LysoPC: lysophosphatidylcholine; LysoPE: lysophosphatidylethanolamine.
