R-Limonene Enhances Differentiation and 2-Deoxy-D-Glucose Uptake in 3T3-L1 Preadipocytes by Activating the Akt Signaling Pathway

Figure 3

Impact of LM on differentiation and lipid accumulation in 3T3-L1 adipocytes. (a) Oil Red O stained lipid droplets in control and LM treated adipocytes on day 10. (b) Percentage of lipid accumulation in experimental adipocytes on day 5. (c) Percentage of lipid accumulation in experimental adipocytes on day 10. Experiments were performed in six replicates and repeated three times with the same results. Bars display mean ± SEM and statistical analysis was performed by one-way ANOVA. Different letters, A, B, C, and D, within a column indicate a statistically significant difference between the groups (p < 0.05).

(a)

(b)

(c)