Cordycepin Accelerates Osteoblast Mineralization and Attenuates Osteoclast Differentiation In Vitro

Figure 1

The effect of cordycepin on MC3T3-E1 cell viability and osteogenic differentiation. (a) Cells were treated with cordycepin (0-5 μM) for 24 h. (b) Cells were incubated for 24 h, 48 h, and 72 h in the presence of 0.5 and 1 μM cordycepin. (c) Mineralized osteoblast nodules formation was detected using alizarin red s staining. Cells were cultured in an osteogenic medium containing 0.5 and 1 μM cordycepin for seven and fourteen days. (d) Quantification of alizarin red s staining was performed after extraction with ethylpyridinium chloride. (e) ALP activity was performed after cordycepin treatment under the same conditions as described in (c). Absorbance was measured at 405 nm. The medium was changed every three days. Error bars (a-e) denote mean SD (standard deviation). P < 0.05 versus control, respectively.