Interspecies Anticancer and Antimicrobial Activities of Genus Solanum and Estimation of Rutin by Validated UPLC-PDA Method

Table 10

Robustness of the developed UPLC method (20 μg/mL, of rutin, mean ± SD, n = 3).

Optimization conditions	Peak area for 20 μg/mL of rutin
	Mean ± SD	% RSD	Rt
Wavelength ( = 332 nm)
(330 nm)	50.253 ± 2.250	4.477	4.185
(332 nm)	49.972 ± 2.477	4.956	4.165
(334 nm)	49.649 ± 2.397	4.827	4.176
Mobile phase flow rate (0.18 ± 0.1 mL/min)
(0.17 mL/min)	54.356 ± 2.528	4.650	4.181
(0.18 mL/min)	50.440 ± 2.366	4.690	4.172
(0.19 mL/min)	46.187 ± 2.269	4.912	4.167