The Effect of Volatile Oil from Vernonia anthelmintica Seeds on Melanin Synthesis in B16 Cells and Its Chemical Analysis by GC-QTOF-MS

Table 1

Volatile compositions in AVO.

	Compound	RT(min)	Possible formula	RI	Content (%)
1	α-Thujene	8.68		1029	6.57
2	Toluene	9.22		1049	0.08
3	Camphene	9.92		1075	0.17
4	Sabinene	11.34		1124	18.15
5	β-Thujene	11.56		1131	1.08
6	α-Phellandrene	12.71		1169	0.53
7	α-Terpine	13.33		1189	0.22
8	Limonene	13.92		1209	4.92
9	Eucalyptol	14.21		1219	1.23
10	Methoxyacetic acid,4-tetradecyl ester	14.76		1239	0.11
11	γ-Terpinene	15.22		1255	0.48
12	o-Cymene	15.94		1281	0.47
13	Terpinolene	16.24		1292	0.47
14	Isomenthone	20.5		1481	0.28
15	Copaene	21.00		1507	0.22
16	Linalyl acetate	21.64		1543	0.94
17	α-Gurjunene	21.80		1552	0.47
18	Fenchol	22.53		1593	0.17
19	β-Elemene	22.6		1596	0.43
20	cis-Caryophyllene	22.83		1610	0.45
21	L-terpinen-4-ol	22.92		1615	0.99
22	Caryophyllene	23.14		1628	23.73
23	dl-Menthol	23.42		1645	0.83
24	Ginsinsene	24.16		1689	0.86
25	4-epi-α-Acoradiene	24.32		1699	4.98
26	α-Terpinyl acetate	24.42		1705	1.38
27	Chamigren	24.52		1711	0.51
28	β-Selinene	24.6		1716	0.22
29	Valencene	24.96		1738	0.20
30	Eremophilene	25.06		1743	0.09
31	Bicyclogermacrene	25.18		1750	0.18
32	δ-Selinene	25.22		1753	0.11
33	Piperitone	25.3		1758	0.26
34	(-)-Carvone	25.37		1762	0.46
35	δ-Cadinene	25.62		1777	0.33
36	Octadecane	25.81		1788	0.20
37	Cuminal	26.22		1813	0.70
38	α-Terpinen-7-al	26.46		1827	0.14
39	Anethole	26.85		1849	3.44
40	2-Methyloctadecane	27.51		1888	0.21
41	Isosafrole	27.77		1903	0.21
42	Alloaromadendrene oxide-(2)	29.74		2015	0.27
43	Caryophyllene oxide	29.96		2026	1.39
44	Globulol	30.96		2081	0.35
45	Phenol, 2-methoxy-4-propyl-	31.83		2128	0.16
46	Thunbergene	32.56		2167	0.11
47	Thymol	32.95		2187	5.29
48	Verticiol	33.27	C20H34O	2205	0.30
49	Hexadecanoic acid, methyl ester	33.45		2214	0.11
50	Carvacrol	33.58		2221	1.62
51	Isophyllocladen	33.88		2236	1.25
52	Cembrene A	34.69		2279	1.09
53	2,4-Di-tert-butylphenol	35.2		2305	0.17
54	α-Pinacene	36.46		2371	1.57
55	Dill apiol	36.66		2382	0.33
56	8,11,13-triene-18-Norabieta	37.94		2446	0.16
57	3,3,4,5,5,8-Hexamethyl-3,5,6,7-tetrahydro-S-indacen-1(2H)-one	39.17		2507	0.20
58	γ-Thujaplicine	40.29		2556	0.19
59	Methyl dehydroabietate	43.25		2673	1.22
60	Kolavenol	46.26		2771	0.34
61	9-Ethyl-10-methylanthracene	46.50		2778	0.10
62	10,18-Bisnorabieta-5,7,9(10),11,13-pentaene	47.56		2809	0.19
63	Retene	48.65		2836	1.12
64	n-Hexadecanoic acid	51.02		2895	0.15
	Total				95.15