Optimized Extract from Corylopsis coreana Uyeki (Hamamelidaceae) Flos Inhibits Osteoclast Differentiation

Figure 2

RANKL-mediated osteoclastogenesis was suppressed by C. coreana Uyeki flos extracts, and the extracts had no cytotoxic effect. (a) BMMs were cultured for 4 days in 0.1% DMSO (control vehicle) or the indicated concentrations of C. coreana Uyeki flos extract with RANKL (10 ng/mL) and M-CSF (30 ng/ml). Multinucleated cells were fixed (3.7% formalin), permeabilized (0.1% Triton X-100), and stained with the TRAP solution. (b) Mature TRAP-positive multinucleated osteoclasts (MNCs) were photographed under an optical microscope. TRAP-positive MNCs (nuclear number > 3) were counted. ; . (c) The effect of C. coreana Uyeki flos extracts on the viability of BMMs was measured by the CCK-8 assay.

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