Simultaneous Evaluation of the Influence of Panax ginseng on the Pharmacokinetics of Three Diester Alkaloids after Oral Administration of Aconiti Lateralis Radix in Rats Using UHPLC/QQQ-MS/MS
Table 5
(a) The stability at 4°C and room temperature of detected methods.
Name
QC conc.
4°C temperature stability (24 h)
Room temperature stability (24 h)
(ng/mL)
Concentration (ng/mL)
RSD (%)
Accuracy (%)
Concentration (ng/mL)
RSD (%)
Accuracy (%)
Aconitine
0.40
0.40 ± 0.01
2.16
99.65 ± 2.16
0.40 ± 0.01
2.51
100.49 ± 2.52
4.00
3.96 ± 0.4
10.10
98.98 ± 9.99
3.85 ± 0.13
3.38
96.29 ± 3.26
8.00
8.13 ± 0.1
1.27
101.57 ± 1.29
7.81 ± 0.36
4.64
97.66 ± 4.54
Mesaconitine
0.40
0.40 ± 0.03
8.71
100.28 ± 8.73
0.39 ± 0.03
7.21
96.34 ± 6.95
4.00
3.88 ± 0.37
9.47
97.02 ± 9.18
3.75 ± 0.11
2.95
93.68 ± 2.77
8.00
7.95 ± 0.37
4.59
99.43 ± 4.57
8.06 ± 0.38
4.74
100.72 ± 4.78
Hypaconitine
0.40
0.39 ± 0.01
2.41
97.44 ± 2.35
0.40 ± 0.01
2.51
100.49 ± 2.52
4.00
4.20 ± 0.22
5.23
104.96 ± 5.49
3.95 ± 0.17
4.35
98.72 ± 4.30
8.00
8.09 ± 0.39
4.76
101.13 ± 4.82
8.05 ± 0.29
3.58
100.64 ± 3.60
(b) The stability at freeze-thaw of detected methods.
