ROS-Mediated Mitochondrial Pathway is Required for Manilkara Zapota (L.) P. Royen Leaf Methanol Extract Inducing Apoptosis in the Modulation of Caspase Activation and EGFR/NF-κB Activities of HeLa Human Cervical Cancer Cells

Table 2

Treatment of Manilkara zapota leaf methanol extract (1.56-200 μg/mL) on selected cancer cell lines for 24, 48, and 72 h evaluated by MTT and LDH assays.

Cancer cell lines		MTT (μg/mL)			LDH (μg/mL)
	24 h	48 h	72 h	24 h	48 h	72 h
HT-29	93.27 ± 17.19	89.29 ± 6.01	69.12 ± 8.10	90.33 ± 15.79	85.99 ± 4.87	76.22 ± 5.39
HCT-116	90.14 ± 14.23	87.33 ± 9.29	83.17 ± 9.92	93.22 ± 9.03	90.12 ± 9.77	88.11 ± 11.69
HeLa	89.29 ± 18.20	59.23 ± 10.33	23.87 ± 5.02	87.33 ± 14.98	80.44 ± 11.65	25.76 ± 8.93
HGT-1	80.11 ± 10.19	72.04 ± 5.23	49.44 ± 10.62	65.20 ± 14.27	62.11 ± 6.29	59.89 ± 10.27
HepG2	97.29 ± 3.26	83.95 ± 9.20	73.02 ± 9.33	89.45 ± 16.82	83.03 ± 5.35	77.04 ± 9.93

HCT-116: human colon carcinoma, HeLa: human cervical cancer, HepG2: human hepatocellular carcinoma, HGT-1: human gastric adenocarcinoma, HT-29: human colorectal adenocarcinoma, LDH: lactate dehydrogenase, and MTT: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide.
Values are reported as mean ± SD (n = 3). Value with different superscript letter in the same row for their respective assay indicates significant difference by Tukey’s test (P < 0.05). In MTT assay, treatment with Manilkara zapota leaf methanol extract for 72 h (69.12 ± 8.10 μg/mL) significantly inhibited the proliferation of HT-29 cells compared to 24 h (93.27 ± 17.19 μg/mL) (P < 0.05), whereas, in LDH assay, there was a significant effect of the cytotoxic activities of Manilkara zapota leaf methanol extract in HT-29 cells incubated for 72 h (76.22 ± 5.39 μg/mL) compared to those incubated for 24 h (90.33 ± 15.79 μg/mL) or 48 h (85.99 ± 4.87 μg/mL) (P < 0.05).