Development of Novel Photosensitizer Using the Buddleja officinalis Extract for Head and Neck Cancer

Figure 1

Schematic of LED irradiation machinery and tools. LED irradiation machinery and tools for in vitro testing were built in a CO₂-humidified chamber. (a) LED irradiation machinery and tools consist of CO₂ incubator and controller: (A) CO₂ incubator door, (B) incubator display window, (C) 625 nm LED light source, (D) water tank, (E) timer, (F) wavelength adjuster, (G) main power switch, and (b) LED investigation of cell culture dishes. For a uniform light energy distribution, the distance between the cell culture medium and the test board was adjusted to 25 mm, so that the LED was designed to illuminate the entire bottom of the Petri dish. The top view of cell culture dish clearly shows high transparency of cell culture containers to LED light. (c) Hardware control system. The controller sets the incubation time and fluorescence. The blue part is the timer part which turns the output signal on/off. The yellow part is the adjuster for adjusting the wavelength of the LED, the orange part is the power meter, and the red part is the main power switch. The total wattage is 270 W, the voltage variable is 0 ~ 30 V, the current variable is 0 ~ 9 A, the output channel for total experiment is 18 channels, and 18 experiments are designed to be carried out simultaneously.

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