Development of Novel Photosensitizer Using the Buddleja officinalis Extract for Head and Neck Cancer

Figure 6

Effects of BO-PDT on cell death mechanism. (a) The protein levels of ATG5, LC3-II, PARP-1, Bax, and Bcl-2 over time. Equal protein loading was confirmed by the antibody reaction to GAPDH. (b) Summary of the effects of BO-PDT on the expression of ATG5, LC3-II, PARP-1, Bcl-2, and Bas over time. Protein levels shown in (a) were measured by densitometry, and their relative levels were compared with the untreated control. (c) Bax/Bcl-2 protein ratio was quantified. P < 0.05 and P < 0.01 versus nontreated control (0 h). (d) Caspase-3, caspase-8, and caspase-9 activity was measured by colorimetric protease assays.

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