In Vitro Antidiabetic Effects of Isolated Triterpene Glycoside Fraction from Gymnema sylvestre

Figure 4

Effects of TG on β-cells viability. (a) MTT was performed to study the cytotoxic effects of TG. MIN6 cells were treated with different concentrations of TG and the cell viability was studied. (b) To study the ROS effect of GS extracts on MIN6 cells, treatment was carried out at concentrations 20, 35, 50, and 100 μg levels. H₂O₂ was used as positive control. Fluorescent microscopy images showing the effect of TG on MIN6 cells, where (c) shows positive control (hydrogen peroxide), (d) shows TG treated cells (50 μg), and (e) shows TG treated cells (100 μg). Green regions indicate uptake of acridine orange, showing live β cells. Orange-red color displays apoptosis/dead cells. All values were represented as mean± SD. Experiments were performed in triplicate. Analysis was performed using two-way ANOVA. Representing significance at p<0.05.

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