Subinhibitory Concentrations of Prim-O-Glucosylcimifugin Decrease the Expression of Alpha-Hemolysin in Staphylococcus aureus (USA300)

Figure 6

The situation of live and dead cells in the A549 human alveolar epithelial cell coculture with S. aureus USA300 treated with different concentrations of prim-O-glucosylcimifugin. Live/dead reagent-stained A549 was observed with fluorescent imaging (100×), using calcein AM and ethidium homodimer-1 (EthD-1), respectively. (a) Uninfected A549 cells; (b) A549 cells was infected by S. aureus USA300 with 32 μg/ml of POG; (c) A549 cells was infected by S. aureus USA300 with 16 μg/ml of POG; (d) A549 cells was infected by S. aureus USA300 with 8 μg/ml of POG; (e) A549 cells was infected by S. aureus USA300 with 4 μg/ml of POG; (f) A549 cells was infected by S. aureus USA300 without POG.

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