HO-1 Induction by Selaginella tamariscina Extract Inhibits Inflammatory Response in Lipopolysaccharide-Stimulated RAW 264.7 Macrophages

Figure 5

Induction of Nrf2/HO-1 signaling pathway by STE. (a) Immunofluorescence images of the nuclear translocation of Nrf2 induced by STE. RAW 264.7 cells were treated with STE (300 μg/mL) for 3 h. (b) Induction of HO-1 by STE. RAW 264.7 cells were treated with various concentrations of STE (10, 100, or 300 μg/mL) for 18 h, lysed, and western blotted. (Significant vs. vehicle-treated control, < 0.05 and < 0.01). (c) Blocking of the NO inhibitory effect of STE on LPS-induced NO production by SnPP. RAW 264.7 cells were pretreated with STE (300 μg/mL) for 1 h in the presence or absence of SnPP (50 μM, 30 min) and then stimulated with LPS (1 μg/mL) for 18 h. ( < 0.001).

(a)

(b)

(c)