Vascular Protection by Ethanol Extract of Morus alba Root Bark: Endothelium-Dependent Relaxation of Rat Aorta and Decrease of Smooth Muscle Cell Migration and Proliferation

Figure 4

A straight wound was made on confluent VSMCs cells in 96-well plates by a commercial Wound Maker. After washing VSMCs with PBS, cells were treated with or without MAE and/or PDGF-BB and incubated in IncuCyte machine for 48 hours. Photographs of the wounded area were scanned immediately at time 0 hours and later at 24 hours and 48 hours after injury (a). The wound density in the migration zone after 48 hours was calculated in terms of percentage with respect to initial wound area filled by migrated VSMCs. Graphs are presented as mean ± SEM. P<0.01; P<0.001, significant difference versus control (PDGF-BB alone).

(a)

(b)