Shenqi Fuzheng Injection Reverses Cisplatin Resistance through Mitofusin-2-Mediated Cell Cycle Arrest and Apoptosis in A549/DDP Cells

Figure 4

SFI reverses the cisplatin resistance of A549/DDP cells. (a) Direct cytotoxic effect of SFI on A549/DDP cells. A549/DDP cells were treated with various concentrations (0, 0.4, 0.8, 1.6, 3.2, 6.4, 12.8, 25.6, and 51.2 mg/mL) of SFI for 26 hours. The IC₅, IC₁₀, and IC₂₀ values were 2, 3.78, and 35.18 mg/mL, respectively. Each data point represents the mean ± standard deviation of results from four individual measurements (: P < 0.05; : P < 0.01). (b) Cocytotoxic effect of cisplatin and SFI on A549/DDP cells. A549/DDP cells were pretreated with IC₅, IC₁₀, and IC₂₀ of SFI (2, 3.78, and 35.18 mg/mL) for 2 hours and then 40 μg/mL of cisplatin (about the concentration of IC₂₀) was added for another 24 hours. The cell viabilities were 68.9%, 47.4%, and 23.2%, respectively.

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