Shenqi Fuzheng Injection Reverses Cisplatin Resistance through Mitofusin-2-Mediated Cell Cycle Arrest and Apoptosis in A549/DDP Cells

Figure 6

Cotreatment with cisplatin and SFI induces cell apoptosis in A549/DDP cells. A549/DDP cells were pretreated with various concentrations (2, 3.78, and 35.18 mg/mL) of SFI for 2 hours and then exposed to cisplatin (40 μg/mL) for another 24 hours. (a) Apoptosis determined by Hoechst staining. “→” shows apoptosis cells with nuclear condensation. (b) Apoptosis determined by Annexin V-FITC/PI staining. Each cytogram consists of data showing live cells (PI- and FITC-negative) in Q3; early apoptotic population (FITC-positive) in Q4; mid- to late-stage apoptosis (PI- and FITC-positive) in Q2; and necrotic/end-stage apoptotic cells (PI-positive and FITC-negative) in Q1. (c) Cell lysates were prepared and subjected to immunoblotting with antibodies to cleaved caspase 3, cleaved-PARP, Bcl-2, Bax, and β-actin. Data are presented in the format of mean ± standard deviation of three independent experiments (: P < 0.05, versus the cisplatin group; : P < 0.01, versus the cisplatin group).

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