Effect of Qufengtongluo Decoction on PI3K/Akt Signaling Pathway in the Kidney of Type 2 Diabetes Mellitus Rat (GK Rat) with Diabetic Nephropathy

Figure 1

HE staining, Masson staining, PASM staining, and 24 hr urinal protein. Histological changes of blank group (), sham-operated group (), and QFTL group () were observed by HE staining, Masson staining, PASM staining. When the Masson and PASM staining were quantified, three fields of each section were randomly chosen. Mean positive rate of these three fields was calculated to stand for the positive rate of whole sample. (a) Glomerular pathological changes of blank group (glomerular hypertrophy, angiotelectasis, mesangial cell proliferation, mesangial matrix expansion, glomerular sclerosis, narrowed Bowman’s capsule, vacuolar degeneration of tubular epithelial cells, tube casts, inflammatory cell infiltration, and fibrous tissue proliferation). (b) Glomerular pathological changes of sham-operated group (glomerular hypertrophy, angiotelectasis, mesangial cell proliferation, mesangial matrix expansion, Bowman’s capsule narrowing, vacuolar degeneration of tubular epithelial cells, inflammatory cell infiltration, and fibrous tissue proliferation). (c) Glomerular pathological changes of QFTL group (glomerular hypertrophy, angiotelectasis, mesangial cell proliferation, mesangial matrix expansion, Bowman’s capsule narrowing, multifocal renal tubular epithelial cell vacuolar degeneration, and derangement of kidney tubules). (d) Masson staining of blank group. (e) Masson staining of QFTL group. (f) PASM staining of blank group. (g) PASM staining of QFTL group. (h) The positive rate of Masson staining: the mean positive rate of blank group was notably higher than that of QFTL group. (i) The positive rate of PASM staining: the mean positive rate of blank group was notably higher than that of QFTL group. (j) The difference value of 24 hr urinal protein.

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