Decoction of Chinese Herbal Medicine Fuzheng Kang-Ai Induces Lung Cancer Cell Apoptosis via STAT3/Bcl-2/Caspase-3 Pathway

Figure 3

FZKA-stimulated activation of caspase-3/9 contributes to the induction of apoptosis in human lung cancer cells. (a) Caspase-3 activity was detected using caspase-3 activity assay kit in H1650 and A549 cells treated with FZKA as indicated doses for 24 h. Data represent means ± SD of three independent experiments. indicates significant difference as compared to the untreated control group (). (b-c) The protein expression levels of caspase-3 and c-caspase-3 were detected by Western blot. β-Actin was used as an internal control. The results were measured by ImageJ software. (d) Caspase-9 activity was detected using caspase-9 activity assay in H1650 and A549 cells treated with indicated doses of FZKA for 24 h. Data represent means ± SD of three independent experiments. indicates significant difference as compared to the untreated control group (). (e) The protein expression levels of caspase-9 and c-caspase-9 were detected by Western blot. β-Actin was used as an internal control.

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