Total Flavone of Rhododendron Improves Cerebral Ischemia Injury by Activating Vascular TRPV4 to Induce Endothelium-Derived Hyperpolarizing Factor-Mediated Responses

Figure 2

Effect of inhibitors of TRPV4, SKca, and IKca on EDHF-mediated dilation and hyperpolarization induced by TFR (n=6 in each group). L-NAME (an inhibitor of nitric oxide synthase), 30 μmol/L; Indo (indomethacin, an inhibitor of cyclooxygenase), 10 μmol/L. (a) Effect of inhibitors of TRPV4 (HC-067047), SKca (Apamin), IKca (TRAM-34) on EDHF-mediated dilation induced by TFR. (b) Effect of inhibitors of TRPV4, SKca, and IKca on EDHF-mediated hyperpolarization of cerebral vascular smooth muscle cells induced by TFR. P<0.05; <0.01 versus IR (L-NAME+Indo). Comparisons were made by unpaired Student’s t-test.

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