Antitrypanosomal Effects of Zanthoxylum zanthoxyloides (Lam.) Zepern. & Timler Extracts on African Trypanosomes

Table 3

Retention times for Kupchan fractions in HPLC.

Peak	(x) RT (min)	Width (min)	Area (mAUs)	(y) Height (mAU)	Area (%)
ZRC
1	2.872	0.1155	61.05810	7.38034	1.7119
2	3.167	0.0989	240.89647	37.04975	6.7541
3	3.373	0.1350	270.91974	37.48990	7.5958
4	3.730	0.1115	2536.17334	337.99835	71.1074
5	4.328	0.1290	115.58913	13.35508	3.2408
6	4.621	0.1279	15.72073	1.79944	0.4408
7	5.301	0.1223	129.75060	15.88849	3.6379
8	5.654	0.1332	33.90483	3.83241	0.9506
9	14.339	0.1536	101.36829	9.34081	2.8421
10	14.693	0.2510	61.30116	3.50620	1.7187
ZRFH
1	2.879	0.1101	32.03850	4.44205	1.4512
2	3.183	0.0999	105.94155	15.86805	4.7988
3	3.377	0.0904	60.97616	9.96817	2.7620
4	3.479	0.0849	35.27485	6.24909	1.5978
5	3.746	0.1102	1726.87097	233.63256	78.2214
6	4.336	0.1189	71.96671	9.13256	3.2598
7	5.318	0.1276	102.20798	11.97620	4.6297
8	14.451	0.1441	72.39410	7.21304	3.2792
ZRFD
1	2.902	0.1293	36.92966	4.47213	1.4285
2	3.185	0.1008	440.68698	64.46280	17.0464
3	3.372	0.0900	94.99347	15.39953	3.6745
4	3.482	0.0933	56.08873	9.30652	2.1696
5	3.743	0.1033	1624.33984	232.98772	62.8316
6	3.927	0.0924	206.64229	32.40942	7.9932
7	4.335	0.1152	41.74596	5.58815	1.6148
8	5.304	0.1243	36.92151	4.52034	1.4282
9	14.357	0.1347	46.87906	5.07797	1.8133
ZRFM
1	2.891	0.1349	63.85921	7.53434	1.6574
2	3.185	0.1042	525.79871	74.60051	13.6468
3	3.374	0.0883	109.30883	18.13418	2.8370
4	3.485	0.0934	75.00216	12.25811	1.9466
5	3.745	0.1082	2825.35791	386.82672	73.3303
6	4.336	0.1164	70.74014	9.34014	1.8360
7	5.306	0.1243	61.64240	7.46639	1.5999
8	14.459	0.1800	121.21284	9.81946	3.1460
ZRWB
1	2.874	0.1237	35.44300	4.41159	3.1619
2	3.174	0.1107	41.06397	5.46061	3.6634
3	3.374	0.1285	43.36354	4.61984	3.8685
4	3.732	0.1124	830.25043	109.60705	74.0683
5	4.324	0.1217	36.01653	4.53515	3.2131
6	5.28 9	0.1306	53.58618	6.15428	4.7805
7	14.342	0.1273	48.23082	5.29576	4.3028
8	14.605	0.2202	32.97150	2.06582	2.9415

Ordinate(x) = absorbance intensity; Abscissa (y) = retention time; ZR=Z. zanthoxyloides LZT (root); ZRC=crude extract of ZR; ZRFH= Z. zanthoxyloides hexane fraction; ZRFD= Z. zanthoxyloides dichloromethane fraction; ZRFM= Z. zanthoxyloides methanol fraction; ZRWB= Z. zanthoxyloides butanol fraction; NA= not applicable.