Adenosine Attenuates LPS-Induced Cardiac Dysfunction by Inhibition of Mitochondrial Function via the ER Pathway

Figure 5

The antagonistic activity of ICI182,780 against adenosine on MMP (or ΔΨm) in LPS-stimulated H9c2 cells as assessed by FCM. H9c2 cells seeded on a 100 mm × 20 mm cell culture flask at a density of 2×10⁵ cells/mL were treated with HQ (0.1 mg/mL) and adenosine (10 μM) for 24 h. In another experiment, the ER nonspecific antagonist Faslodex (ICI182,780, 1 μM) was added 30 min prior to HQ (0.1 mg/mL) and adenosine (10 μM) to evaluate whether the observed effects elicited by HQ and adenosine were mediated by the ER. Cells in each group were collected 24 h later and ΔΨm was assessed.