Chemical Constituents and an Antineuroinflammatory Lignan, Savinin from the Roots of Acanthopanax henryi

Figure 3

Effects of compound 3 on LPS-induced production and expression of pro-inflammatory cytokines and their mRNA, IL-1β (a, d), IL-6 (b, e), and TNF-α (c, f) in BV2 microglial cells. Cells were pretreated with/without the indicated concentrations of compound 3 for 3 h and then stimulated with LPS (1 μg/mL) for 24 h and 6 h, respectively. The concentrations of cytokines were measured by ELISA, and the mRNA levels of the proinflammatory cytokines were analyzed by qRT-PCR. < 0.05, < 0.01, and < 0.001 in comparison with LPS-treated group.

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