Chemical Constituents and an Antineuroinflammatory Lignan, Savinin from the Roots of Acanthopanax henryi

Figure 4

Effects of compound 3 on the LPS-induced activation of MAPK pathways in BV2 microglial cells. Lysates were prepared from cells pretreated with/without the indicated concentrations of compound 3 for 3 h and then with LPS (1 μg/mL) for 30 min. The levels of p-p38, p38, p-ERK, ERK, p-JNK, and JNK were determined by Western blot analysis. Actin was used as a loading control. The experiment was repeated three times, and similar results were obtained.

(a)

(b)

(c)