Yu Ping Feng San Exert Anti-Angiogenesis Effects through the Inhibition of TSLP-STAT3 Signaling Pathways in Hepatocellular Carcinoma

Figure 3

Effects of YPFS on the signal pathways of TSLP/STAT3. (a) Expression levels of TSLP in tumor tissues treated with indicated concentration of YPFS were measured by ELISA. (b) Expression levels of TSLPR in tumor tissues treated with indicated concentration of YPFS were measured by ELISA. (c) The expression of STAT3 and p-STAT3 in tumor tissues treated with indicated concentration of YPFS detected by western blot. (d) ELISA analyzed the expression of TSLP in Hepa1-6 cell of the YPFS treatment. (e) ELISA analyzed the expression of TSLPR in Hepa1-6 cell of the YPFS treatment. (f) The expression of STAT3 and p-STAT3 in Hepa1-6 cell of the YPFS treatment detected by western blot. Quantification of western blot analysis of p-STAT3 and STAT3 levels normalized by the levels of GAPDH. Data are presented as the mean ± standard error of the mean. , , , compared with the vehicle group.

(a)

(b)

(c)

(d)

(e)

(f)