Yu Ping Feng San Exert Anti-Angiogenesis Effects through the Inhibition of TSLP-STAT3 Signaling Pathways in Hepatocellular Carcinoma

Figure 4

Anti-TSLP antibody can attenuate the inhibitory effect of YPFS on angiogenesis and VEGF secretion. (a) Tumor tissues treated with the anti-TSLP antibody and indicated concentration of YPFS (30 g/kg) mice were performed by immunohistochemical staining for detecting vascular marker CD34. The pointed tip indicated a positive result and was observed under a 200x microscope. (b) Expression levels of VEGF in tumor tissues treated with the anti-TSLP antibody and indicated concentration of YPFS (30 g/kg) mice were measured by ELISA. (c) Expression levels of VEGF in Hepa1-6 cell treated with the anti-TSLP antibody and indicated concentration of YPFS (30 mg/mL) mice were measured by ELISA. The tumor tissues were observed with a light microscope at ×200 magnification. Data are presented as the mean ± standard error of the mean. , , compared with the vehicle group.

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