Yu Ping Feng San Exert Anti-Angiogenesis Effects through the Inhibition of TSLP-STAT3 Signaling Pathways in Hepatocellular Carcinoma

Figure 6

Effect of YPFS on TSLP-induced vascularization of HUVEC. (a) MTT assays with HUVEC cells induced by TSLP (100 ng/mL) combined with the antibody of anti-TSLP, anti-TSLPR and indicated concentration of YPFS (10, 30, 50 mg/mL). (b) The expression of PCNA in HUVEC treated with TSLP (100 ng/mL), the anti-TSLP and anti-TSLPR antibody and indicated concentration of YPFS (10, 30, and 50 mg/mL) detected by western blot. (c) The expression of STAT3 and p-STAT3 in HUVEC treated with TSLP (100 ng/mL), the anti-body of anti-TSLP, anti-TSLPR, and indicated concentration of YPFS (10, 30, and 50 mg/mL) detected by western blot. (d) HUVEC tube formation treated with TSLP (100 ng/mL), the anti-TSLP and anti-TSLPR antibody, and indicated concentration of YPFS (10, 30, and 50 mg/mL) (100x magnification). Quantification of western blot analysis of p-STAT3 and STAT3 levels normalized by the levels of GAPDH. Data are presented as the mean ± standard error of the mean. , , , compared with the vehicle group.

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