Network Pharmacology-Based Study on the Mechanism of Bushen-Jianpi Decoction in Liver Cancer Treatment

Figure 7

Effects of BSJPD-medicated serum, licochalcone A, alisol B, and hederagenin on the expression of PI3K-Akt-mTOR pathway components and apoptosis-related pathway molecules in HepG2 cells. (a) Western blot analysis of the effects of BSJPD-medicated serum on the expression of PI3K, p-Akt, Akt, p-mTOR, mTOR, Bcl-xL, BAD, p53, and CASP3. (b) Western blot analysis of the effects of licochalcone A, alisol B, and hederagenin on the expression of PI3K, p-Akt, Akt, p-mTOR, mTOR, Bcl-xL, BAD, p53, and CASP3. Notes. BSJPD, Bushen-Jianpi decoction; LCLA, licochalcone A; ALSB, alisol B; HDG, hederagenin; control, cultured with FBS; rat control, cultured with rat serum. p < 0.05 and p < 0.01 compared with the control group. These groupings of blots are cropped from the same gel or different gels. All full-length blots are included in Supplementary Figures 3 and 4.

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