An Ancient Chinese Herbal Decoction Containing Angelicae Sinensis Radix, Astragali Radix, Jujuba Fructus, and Zingiberis Rhizoma Recens Stimulates the Browning Conversion of White Adipocyte in Cultured 3T3-L1 Cells

Figure 2

DBT₁₁₅₅ decreases lipid accumulation. 3T3-L1 adipocytes were cultured to 10 days of differentiation and then applied with cocktail (1.8 μM of rosiglitazone and triiodothyronine), or different concentrations of DBT₁₁₅₅ (1 mg/mL of DBT labeled as DBT-H; 0.125 mg/mL of DBT labeled as DBT-L) or ZRR (1 mg/mL of ZRR labeled as ZRR-H; 0.125 mg/mL of ZRR labeled as ZRR-L) for another 3 days. (a) Oil Red O staining was to measure lipid accumulation. Bar = 50 μm. (b) The amount of stained lipid was quantified at 490 nm absorbance. Data were expressed as mean ± SEM of the percentage of change as compared with control, where n = 3; p < 0.05 (); p < 0.01 (); p < 0.001 ().

(a)

(b)