An Ancient Chinese Herbal Decoction Containing Angelicae Sinensis Radix, Astragali Radix, Jujuba Fructus, and Zingiberis Rhizoma Recens Stimulates the Browning Conversion of White Adipocyte in Cultured 3T3-L1 Cells

Figure 3

DBT₁₁₅₅ triggers browning mRNA expressions of WAT markers. 3T3-L1 adipocytes were cultured to 10 days of differentiation. Then, the cultures were applied with cocktail or different concentrations of DBT₁₁₅₅ (DBT-H: 1 mg/mL of DBT; DBT-L: 0.125 mg/mL) with/without cotreatment of BAMPTA-AM (10 μM) for another 3 days. Total RNAs were isolated and reverse-transcribed to cDNA for PCR analysis. The mRNA levels of PPARγ, PGC1α, and UCP1 were determined by the Ct-value method and normalized by the house keeping gene 18S rRNA. Data were expressed as mean ± SEM as compared with control, setting as 1 here, where n = 3; p < 0.05 (); p < 0.01 (); p < 0.001 ().