An Ancient Chinese Herbal Decoction Containing Angelicae Sinensis Radix, Astragali Radix, Jujuba Fructus, and Zingiberis Rhizoma Recens Stimulates the Browning Conversion of White Adipocyte in Cultured 3T3-L1 Cells

Figure 4

DBT₁₁₅₅ triggers protein levels of browning WAT markers. 3T3-L1 adipocytes were treated with cocktail or different concentrations of DBT₁₁₅₅ (DBT-H: 1 mg/mL of DBT; DBT-L: 0.125 mg/mL) with/without cotreatment of BAMPTA-AM (10 μM) for another 3 days after 10 days of differentiation, as in Figure 2. The translational levels of PPARγ (~58 kDa), PGC1α (~100 kDa), and UCP1 (~30 kDa) were detected by immunoblot analysis by specific antibodies. GAPDH (~38 kDa) served as an internal control. Quantification of target protein expression was calculated by a densitometer (lower panel). Data were expressed as mean ± SEM as compared with control, setting as 1 here, where n = 3; p < 0.05 (); p < 0.01 (); p < 0.001 ().