An Ancient Chinese Herbal Decoction Containing Angelicae Sinensis Radix, Astragali Radix, Jujuba Fructus, and Zingiberis Rhizoma Recens Stimulates the Browning Conversion of White Adipocyte in Cultured 3T3-L1 Cells
Figure 5
DBT1155 stimulates Ca2+-AMPK pathway. 3T3-L1 adipocytes were pretreated with medium (a) or BAMPTA-AM (10 μM) (b) for 3 hours and then were labeled with fluorescent Ca2+ indicator Fluo-4 AM for half an hour. Fluorimetric measurement was performed after the treatment of different concentrations of DBT1155 decoctions, as in Figure 2. A23187 (100 nM) served as a control. The amounts of Ca2+ were evaluated by measuring the fluorescence intensity (left panel). Micrographs were taken by a confocal microscope; Bar = 100 μm. Differentiated cells were subjected to the phosphorylation assay. Phospho-AMPK (P-AMPK, ~ 60 kDa) and total AMPK (T-AMPK, ~ 60 kDa) were revealed by using specific antibodies (right panel). Representative photos were shown, n = 4.