Asparagus Polysaccharide Suppresses the Migration, Invasion, and Angiogenesis of Hepatocellular Carcinoma Cells Partly by Targeting the HIF-1α/VEGF Signalling Pathway In Vitro
Figure 2
ASP inhibited the migration and invasion of HCC cells. (a) Wound healing assay was performed to test the effect of ASP (0, 2.5, 5, and 10 mg/ml, for 24 h) on migration in SK-Hep1 and Hep-3B cells. The same point of view was selected to take photographs at 0 h and 24 h. Histogram presented the migration distance relative to that of the control group for 24 h after scratching. Data were graphed using GraphPad Prism 5.02. , p<0.05 compared with the control group. (b) Transwell invasion assay was performed to test the effect of ASP (0, 2.5, 5, and 10 mg/ml, for 72 h) on invasion in SK-Hep1 and Hep-3B cells. Cells on the lower surface of the transwell membrane were stained with 0.1% crystal violet. Histogram presented the average number of invaded cells per field. Data were graphed using GraphPad Prism 5.02. , p < 0.05 compared with the control group.