Hydroxysafflor Yellow A Inhibits TNF-α-Induced Inflammation of Human Fetal Lung Fibroblasts via NF-κB Signaling Pathway

Figure 1

Effect of HSYA on TNF-α-induced proliferation and inflammatory response of MRC-5 cells. (a), (e), and (h), MTT assays were used to detect the effect of TNF-α or/and HSYA on the proliferation of MRC-5 cells; (b), (f), and (i), quantitative RT-PCR was used to detect the mRNA expression levels of IL-1β, IL-6, and TGF-β1; (c), (g), and (j), IL-1β, IL-6, and TGF-β1 protein expression levels were determined by western blot analysis; (d); high-pressure liquid chromatography analysis of HSYA; HSYA retention time was 20 min; HSYA, hydroxysafflor yellow A; ; ; .

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