Hydroxysafflor Yellow A Inhibits TNF-α-Induced Inflammation of Human Fetal Lung Fibroblasts via NF-κB Signaling Pathway

Figure 2

HSYA competes with TNF-α to bind with TNFR1. (a) MTT assay was used to detect the proliferation of TNF-α stimulated MRC-5 cells; (b) quantitative RT-PCR was used to detect mRNA expression levels of IL-1β, IL-6, and TGF-β1; (c-d), western blot was used to detect the protein expression levels of IL-1β, IL-6, and TGF-β1; (e) immunofluorescence was used to detect the colocalization of TNF-α and TNFR1; HSYA, hydroxysafflor yellow A; ; .

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