Proteome Profiling of Membrane-Free Stem Cell Components by Nano-LS/MS Analysis and Its Anti-Inflammatory Activity

Figure 9

Effect of MFSCC on LPS-induced production NO and proinflammatory mediators in RAW 264.7 cells. (a) NO production was measured in RAW264.7 cells treated with MFSCC at concentrations of 0, 0.4, 0.8, 1, 1.5, and 2 μg/mL for 24h with LPS (1 μg/mL) pretreatment for 1h. (b) Effect of MFSCC on LPS-induced iNOS and COX-2 protein expression levels in RAW264.7 cells. Cells were pretreated with LPS (1μg/mL) for 1h and then treated with MFSCC at concentrations of 0, 1, and 2 μg/mL for 24 h. β‐actin was used as a loading control. The values are expressed as the mean ± SD of three independent experiments (###p < 0.05 vs. untreated; ∗∗p < 0.05 vs. LPS treated).

(a)

(b)