In Vivo Study on the Effects of Xiaoaiping on the Stemness of Hepatocellular Carcinoma Cells

Table 1

Sequence of the primers used in qPCR.

Primer name	Forward sequence5′-3′	Reverse sequence3′-5′	Products (bp)
PTCH	TGGAACGAGGACA AAGCGG	AGGCATAGGCGAGCATGAGTAA	202
SMO	TCTCGGGCAAGACCTCCTACT	CGCACGGTATCGGTAGTTCTT	146
GLI1	CCAAGCACCAGAATCGGACC	TTTGGTCACATGGGCGTCAG	140
GLI2	AGGGATGACTGTAAGCAGGAGG	CGGCACACAAACTCCTTCTTCT	152
GLI3	GCACTAAGCGTTACACAGACCCA	CTTCTCTGCCTTGACGGTTTTC	257
SHH	CTGCTGGTATGCTCGGGACT	CATTGGGGATAAACTGCTTGTAG	106
DHH	GATGACCGAGCGTTGTAAGGA	GTTATCAGCTTTGACCGACACG	277
FU	TTGCTGCCCAGTTGGTGTCA	TGTGGTCGTATGGTCGCTCCT	225
GAS1	ATCTGCGAGTCGGTCAAGGA	TGCGCTGCTCGTCATCGTA	133
Cdon	CTTTTCCAGCCGTCCAATAACT	GTGCCACTGCTTTGAACCTTCT	205
BOC	CATCACTGCCCTTAACAACCAC	TGTTTCCCTCATCCACTTCAATC	158
HH	TCACCGTCTGGCACCCTAGT	CATGGCTCCTCTTGAACCCTG	120
HHIP	GAGAAGGTGCCTGAATGGGAAC	GGTGAGTGGAACAGGCTTTGA	270
SUFU	GCCTTCGCTTCGCTCTTTC	AGGCCGAAGCTGATGTAGTGC	212
MST1	CGGGTCCCAGTAGCCAAGAT	GTGTCATTACCCGTACCTTTGG	191
MST2	ACCATCTGCCTTAGGAACGGA	TAATTGCGACAACTTGACCGG	223
LATS1	CCTATTAATGCCAGCATGAAACC	CGTTGCTAGGGTGAGCTTGA	220
LATS2	GAGCAGATTGTGCGGGTCATT	TGGTGGTAGGACGCAAACGA	116
SAV1	GAATGCCACAGAATCAGGGGA	GATGCCTGTATTGGGCCTTCTT	291
MOB1A	AGTGGGAATCTGAGACAAGCTG	GGTGCAGAACATTTGATTGGCT	224
MOB1B	TGGGCAGATGGAACGAACA	GAAGCTGGATCACAGGGTCAA	232
YAP	GAGTTAGCCCTGCGTAGCCA	GGCAGGGTGCTTTGGTTGATA	272
TAZ	CCTGAAACTCCGCCACATCT	CTGGTAGACGCCATCTCCTTTC	235
Notch1	GTCAACGCCGTAGATGACC	TTGTTAGCCCCGTTCTTCAG	101
Notch2	ACTGTGAGGAGCAACTCGAT	TCCACTTCATACTCACAGTTGA	133
Notch3	TGACCGTACTGGCGAGACT	CCGCTTGGCTGCATCAGCA	67
Notch4	AACTCCTCCCCAGGAATCTG	CCTCCATCCAGCAGAGGTT	168
JAG1	GCCAGGAAGTTTCAGGGAGA	GCTGGAGACTGGAAGACCGA	264
JAG2	CTGACTGCCGCATCAACATC	GCTACAGCGATACCCGTTGA	92
DLL1	TCTCCTGATGACCTCGCAACA	TCACACACGAAGCGGTAGGAG	149
DLL3	CACTCAACAACCTAAGGACGCA	CGAGGAAGGGTAGGGAAAAAG	212
DLL4	GACCTCTCCACAGACACCTTTG	TCCACTTCCAGCTCCTTCTTCT	299
HES1	ATTCTGGAAATGACAGTGAAGCAC	CACCTCGGTATTAACGCCCTC	168
HES5	GAAAAACCGACTGCGGAAGC	GACGAAGGCTTTGCTGTGCT	184
NGN	CAGATTTGGTCCCATTTGTGAG	ATGGCAACACTACATCCTGACC	279
Hey1	GAAGCAGGTAATGGAGCAAGGA	GAAGCGTAGTTGTTGAGATGCG	258
HEY2	AAGGCTACTTTGACGCACACG	GAGATGAGACACAAGCCGCAC	146
HES1	ATTCTGGAAATGACAGTGAAGCAC	CACCTCGGTATTAACGCCCTC	168
DV1	AGAAGTCAGCTCTTGCCTCAGTT	ATCTCATCAGTAGCACGACGAAG	256
AXIN	TCTGGATACCTGCCGACCTTA	TCTGCTGCTCGCTGTCGTT	271
APC	CAAAACTGGAAACTGAGGCATCT	ACTCTCCAGAACGGCTTGATACA	225
β-catenin	GCCAAGTGGGTGGTATAGAGG	GGGATGGTGGGTGTAAGAGC	192
GSK3B	GTTAGCAGAGACAAGGACGGCA	GCAATACTTTCTTGATGGCGAC	183
GBP	AGGTGGCTCCTGACGCTAA	CAGGCTGGAAGGGAAAGACA	180
TCF	GAGTATGCCTACCTCAAAGCCA	AGCCGCTTGATCTTCCCTG	80
FRZB	ACGGAAACTGTAGAGGGGCA	CAGTGTCCCGTGGAATGTTTAC	204
c-Myc	TGCTGCCAAGAGGGTCAAGT	GCTCCGTTTTAGCTCGTTCC	160
PKC	GCTTATGCTGTCATGTCCCGG	GATGATGAGGACTCCCCCCA	120
RHOA	ATTGTTGGTGATGGAGCCTGTG	GTGGGCACATACACCTCTGG	83
ILK	GCAGTGAATGAACACGGGAAT	CACCAGGTCCTCTGCCACTT	78
CD24	GCTCCTACCCACGCAGATTT	GGTGGCATTAGTTGGATTTGG	109
CD13	CCGACATTGACAAGACTGAGCT	ACCTTTCTGACATTGCCCTCC	175
CD44	TCCAACACCTCCCAGTATGACA	CTTTCTGGACATAGCGGGTG	151
CD90	CGCTCTCCTGCTAACAGTCTTG	GTTCGGGAGCGGTATGTGTG	213
CD45	TGAGGAGCAAGGAAGCCAATC	GCCACCAACTGAAGGCTGAAC	77
ALDH1	TGCCGACTTGGACAATGCT	GCCTCCTCCACATTCCAGTTT	277
ICAM1	CCGTTGCCTAAAAAGGAGTTGC	TGGCAGCGTAGGGTAAGGTTC	221
LGR5	CGGGAAACGCTCTGACATACAT	ACTTCTAAAAGCCTGGACGGG	260
CK7	TGAAGAAGGATGTGGATGCTGC	CAGCTCTGTCAACTCCGTCTCAT	119
CK19	AGAATTGAACCGGGAGGTCG	CCTGATTCTGCCGCTCACTA	257
CD34	GCAACATCTCCCACTAAACCCTA	GTCCTTCTTAAACTCCGCACAGC	156
CD47	ACCTCCTTCGTCATTGCCATATT	ATACACGCCGCAATACAGAGAC	89
DLK1	CCCTTTGTGACCAGTGCGTG	ATTCATAGAGGCCATCGTCCAG	183
EpCAM	TAATCGTCAATGCCAGTGTACTTC	AGCCATTCATTTCTGCCTTCAT	101
CD133	TGGCATCTTCTATGGTTTTGTGG	TCCTTGGTAGTGTTGTACTGGGC	159
OCT4	GAGTGAGAGGCAACCTGGAGAAT	ACCGAGGAGTACAGTGCAGTGAA	292
SOX2	GGTTACCTCTTCCTCCCACTCC	CGCTCTGGTAGTGCTGGGAC	140
NANOG	GAGAAGAGTGTCGCAAAAAAGGA	TGAGGTTCAGGATGTTGGAGAGT	163
FOXD3	GCAACTACTGGACCCTGGAC	TAAGCGCCGAAGCTCTGCAT	142
SALL4	CATCAACTCGGAGGAGGACCA	TGATGAGGACAGGTGGATTTTTAGT	275
β-actin	AGTTGCGTTACACCCTTTCTTGAC	GCTCGCTCCAACCGACTGC	171