Cudraxanthone D Regulates Epithelial-Mesenchymal Transition by Autophagy Inhibition in Oral Squamous Cell Carcinoma Cell Lines

Figure 4

CD acts as an autophagy inhibitor in resveratrol-induced autophagy in Ca9-22 cells. (a) Cells were pretreated with 3MA (1 mM) for 2 h and then 25 μM resveratrol and 50 μM CD for 24 h. After treatment, AO-stained cells were observed under a confocal microscope. (b) Autophagy-related protein expression was confirmed by western blot. Each group was treated at the indicated concentrations and times. (c) Relative protein expression levels were normalized to β-actin. Error bars represent mean  standard deviation (SD). versus control, respectively.

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