Brazilian Green Propolis Inhibits Ox-LDL-Stimulated Oxidative Stress in Human Umbilical Vein Endothelial Cells Partly through PI3K/Akt/mTOR-Mediated Nrf2/HO-1 Pathway
Figure 4
EEBP ameliorated oxidative stress in Ox-LDL-stimulated HUVECs at 6 h. (a), mRNA levels of HO-1 and Nrf2. (b), Expression of HO-1 and Nrf2 of total cell lysate in Ox-LDL-stimulated HUVECs. (c), Expression of Nrf2 of nuclear lysate in Ox-LDL-stimulated HUVECs. (d), Expression of Nrf2 of cytoplasmic lysate in Ox-LDL-stimulated HUVECs. (e), Fluorescent micrographs of ROS obtained in Ox-LDL induced HUVECs at 6 h. (f), Quantification of relative quantity of ROS in Ox-LDL induced HUVECs at 6 h. Values represent the relative fluorescent intensity per cell determined by laser scanning confocal microscopy. (g), Fluorescent micrographs of mitochondrial membrane potential obtained in Ox-LDL induced HUVECs at 6 h. (h), Quantification of relative fluorescent intensity per cell determined by laser scanning confocal microscopy. Values are represented as ratio of red to green fluorescence (P < 0.05, P < 0.01 vs Ox-LDL, n=3). Data are means ± S.E.M.