The Effect of Ginsenoside RB1, Diazoxide, and 5-Hydroxydecanoate on Hypoxia-Reoxygenation Injury of H9C2 Cardiomyocytes

Figure 12

GS-Rb1 reduced the apoptosis rate of H9c2 cells during HR compared with nontreated cells. Apoptosis was measured using the TUNEL/DAPI assay. TUNEL staining revealed apoptotic nuclei, DAPI staining showed total nuclei, and MERGE visualizes the ratio of apoptotic nuclei to total nuclei (the apoptosis rate). Representative fluorescent images were obtained using a fluorescent microscope (Olympus, Japan) at a magnification of 200×. HR, hypoxia-reoxygenation; GS-Rb1, ginsenoside Rb1; 5-HD, 5-hydroxydecanoate.