The Effect of Ginsenoside RB1, Diazoxide, and 5-Hydroxydecanoate on Hypoxia-Reoxygenation Injury of H9C2 Cardiomyocytes

Figure 13

Protective effect of GS-Rb1 on the apoptosis rate of H9c2 cells during HR. Apoptosis was measured by Hoechst 33342 staining (light bars) or TUNEL/DAPI assay (dark bars). Column A, control H9c2 cells not subjected to HR. Column B, H9c2 cells subjected to HR without pretreatment showed increased apoptosis compared with nontreated cells. Column C, pretreatment with GS-Rb1 protected H9c2 cells from apoptosis during HR. Column D, simultaneous pretreatment with 5-HD prevented GS-Rb1 from protecting H9c2 cells during HR. Column E, pretreatment with diazoxide reduced the apoptosis rate of H9c2 cells during HR compared with nontreated cells. HR, hypoxia-reoxygenation; GS-Rb1, ginsenoside Rb1; 5-HD, 5-hydroxydecanoate.