The Effect of Ginsenoside RB1, Diazoxide, and 5-Hydroxydecanoate on Hypoxia-Reoxygenation Injury of H9C2 Cardiomyocytes

Figure 9

GS-Rb1 reduces the continuous opening of mPTP in H9c2 cells during HR. The fluorescence intensity is negatively correlated with the opening of mPTP. A, the control cells displayed uniform high-intensity fluorescence. B, the fluorescence intensity of the H9c2 cells during HR decreased significantly compared with control cells. C, pretreatment with GS-Rb1 significantly increases the fluorescence intensity of H9c2 cells during HR compared with nontreated cells. D The protective effect of GS-Rb1 can be partially eliminated by 5-HD. E, Pretreatment with diazoxide also significantly increases the fluorescence intensity of H9c2 cells during HR compared with nontreated cells. Cells were visualized by a fluorescent microscope (Olympus, Japan). Magnification: 200x. HR, hypoxia-reoxygenation; GS-Rb1, ginsenoside Rb1; 5-HD, 5-hydroxydecanoate.