Development of a MD-LC-MS/MS Method to Analyze 3 Bioactive Compounds in Huoxuezhitong Rubber Patch and Application to a Pharmacokinetic Study in Rats

Table 3

Accuracy and precision for the analytes in blank microdialysate (three validation days, six replicates at each concentration level per day) (n=6).

Concentration (ng/mL)	Precision, RSD (%)		Accuracy, RE (%)
	Intra-day	Inter-day	Intra-day	Inter-day
Paeonol
1	5.82	12.4	-11.82	-12.23
20	1.66	5.21	-14.92	-10.61
80	2.51	3.64	-9.65	-7.61
Eugenol
1	4.93	11.76	6.07	-6.54
20	2.52	8.83	-12.42	-12.67
80	1.81	3.67	-5.27	-8.3
Piperine
0.003	13.56	9.8	0.22	0.07
0.1	1.02	7.38	14.00	13.72
4	1.32	10.95	10.96	11.07