Development of a MD-LC-MS/MS Method to Analyze 3 Bioactive Compounds in Huoxuezhitong Rubber Patch and Application to a Pharmacokinetic Study in Rats

Table 4

Stability of the three analytes in blank microdialysate (n = 3).

Concentration (ng/mL)	Room temperature for 12 h	Room temperature for 24 h
	RSD (%)	RSD (%)
Paeonol
1	4.12	12.16
20	2.09	4.1
80	0.28	4.05
Eugenol
1	15.92	11.9
20	3.91	3.51
80	2.71	7.11
Piperine
0.003	13.59	17.55
0.1	1.84	1.30
4	13.51	7.42