Development of a MD-LC-MS/MS Method to Analyze 3 Bioactive Compounds in Huoxuezhitong Rubber Patch and Application to a Pharmacokinetic Study in Rats

<table class="fixed-width table-group" id="tab5"><tr><td><table class="table"><colgroup><col style="width:7.96em"/><col style="width:7.96em"/><col style="width:7.96em"/><col style="width:7.96em"/><col style="width:7.96em"/><col style="width:7.96em"/><col style="width:7.96em"/></colgroup><tr><td class="thead-hr" colspan="7"><hr/></td></tr><tr class="thead"><td class="align_left">Analytes</td><td class="align_center"><svg height="12.0621pt" id="M12" style="vertical-align:-3.2957pt" version="1.1" viewbox="-0.0498162 -8.7664 25.3694 12.0621" width="25.3694pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M614 175C564 76 510 21 408 21C256 21 146 149 146 336C146 488 235 629 402 629C510 629 570 586 597 480L626 488C620 541 614 582 606 638C578 643 510 665 429 665C206 665 44 527 44 316C44 157 153 -15 402 -15C474 -15 558 5 586 11C604 45 629 119 643 165L614 175Z"></path></g><g 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46 340C46 316 72 295 91 295C106 295 117 303 122 319C131 350 139 371 152 384C163 397 178 404 197 404C246 404 281 365 281 293V278C260 262 186 232 124 213C69 194 41 161 41 112C41 47 92 -12 163 -12C195 -12 243 25 283 51C288 35 293 22 308 7C319 -4 339 -12 354 -12L442 39ZM281 87C262 68 226 51 199 51C169 51 129 74 129 126C129 162 151 181 190 198C211 208 259 229 281 240V87Z"></path></g><g transform="matrix(.0091,0,0,-0.0091,19.669,3.132)"><path d="M482 0V30C420 38 407 48 370 103L273 248C308 299 331 332 352 357C387 397 401 404 463 409V439H277V409C318 405 324 400 307 369C290 337 270 307 251 279L194 367C175 396 180 402 220 409V439H18V409C74 401 87 395 117 349L204 213C173 168 147 130 119 95C78 46 67 38 5 30V0H195V30C144 38 143 47 162 79C181 112 202 149 225 181L297 70C314 43 307 35 264 30V0H482Z"></path></g></svg> (min)</td><td class="align_center"><svg height="12.0575pt" id="M14" style="vertical-align:-3.2911pt" version="1.1" viewbox="-0.0498162 -8.7664 48.1839 12.0575" width="48.1839pt" 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transform="matrix(.0091,0,0,-0.0091,35.177,3.132)"><path d="M1000 227C1000 351 905 451 780 451C658 451 590 380 530 279C465 385 388 451 261 451C168 451 38 376 38 210C38 98 121 -12 261 -12C381 -12 448 59 508 160C573 53 652 -12 780 -12C879 -12 1000 64 1000 227ZM485 199C434 109 370 25 271 25C188 25 133 117 133 241C133 355 189 414 249 414C357 414 426 302 485 199ZM903 204C903 70 852 25 790 25C681 25 611 136 552 240C603 330 669 414 768 414C855 414 903 321 903 204Z"></path></g></svg> (min)</td></tr><tr><td class="thead-hr" colspan="7"><hr/></td></tr><tr><td class="align_left">paeonol</td><td class="align_center">12.59±3.29</td><td class="align_center">186.67±48.44</td><td class="align_center">5.02±1.94</td><td class="align_center">336.98±228.04</td><td class="align_center">363.37±53.40</td><td class="align_center">570.07±316.75</td></tr><tr><td class="align_left">eugenol</td><td class="align_center">18.87±7.11</td><td class="align_center">240.00±138.56</td><td class="align_center">7.12±3.81</td><td class="align_center">228.33±161.87</td><td class="align_center">372.20±80.35</td><td class="align_center">466.03±220.48</td></tr><tr><td class="align_left">piperine</td><td class="align_center">1.80±1.22</td><td class="align_center">488.00±150.73</td><td class="align_center">0.88±0.53</td><td class="align_center">336.94±44.44</td><td class="align_center">550.47±66.44</td><td class="align_center">690.20±97.33</td></tr><tr class="table-tr"><td colspan="7"><hr class="tbody-hr"/></td></tr></table></td></tr></table>

<div>Pharmacokinetic parameters of the 2 investigated compounds in male SD rats after transdermal administration Huoxuezhitong rubber patch (mean ± SD, n = 6).</div>

Evidence-Based Complementary and Alternative Medicine

tab5

Table 5

Table 5: Development of a MD-LC-MS/MS Method to Analyze 3 Bioactive Compounds in Huoxuezhitong Rubber Patch and Application to a Pharmacokinetic Study in Rats 

Table 5 | Development of a MD-LC-MS/MS Method to Analyze 3 Bioactive Compounds in Huoxuezhitong Rubber Patch and Application to a Pharmacokinetic Study in Rats 