Chelidonine Induces Caspase-Dependent and Caspase-Independent Cell Death through G_2/M Arrest in the T98G Human Glioblastoma Cell Line

Figure 2

Chelidonine induces caspase-dependent and -independent apoptosis in T98G cells. (a) Cells were pretreated with 50 μM Z-VAD-FMK for 1 h, followed by treatment with 0.6 μM chelidonine for 24 h. (b) Whole T98G cell lysates were subjected to western blot analysis with the indicated antibodies. The arrows indicate bands corresponding to cleaved caspase-9. Arrow: cleaved caspase-9. (c) T98G cells were synchronized by double thymidine inhibition, washed, and then incubated with 0.6 μM chelidonine for indicated periods of time after synchronization. They were then immunostained for AIF (red) and DNA (DAPI; blue). Images were captured using confocal laser scanning microscopy. Magnification, 600X. Scale bar, 10 μm.

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