Chelidonine Induces Caspase-Dependent and Caspase-Independent Cell Death through G2/M Arrest in the T98G Human Glioblastoma Cell Line
Figure 3
Chelidonine induces arrest in T98G cells. T98G cells were seeded in six-well plates and incubated with the indicated concentration of chelidonine for 24 h (a). They were then stained with propidium iodide and analyzed with flow cytometry. (b) The numbers of cells in phase of cell cycle were analyzed using ModFit LT™. (c) T98G cells were treated with 2 mM thymidine for 12 h, the thymidine was removed by washing with PBS (3 times), and fresh media was added to the culture plates for 12 h, after which they were retreated with 2 mM thymidine for 12 h. The G1/0/ arrested cells were then released by PBS washing and the addition of fresh medium containing 0.6 μM chelidonine or DMSO for the indicated periods of time. The cell cycle was analyzed at the indicated time points by PI staining and flow cytometry. The data show the percentages of cells in and phase (c) and sub-G1/0 (d). Error bars represent the standard deviation. The data were analyzed using t-test. p < 0.05, p < 0.01, p < 0.001.