Research Article
Effect of Rhus verniciflua Extract on IgE-Antigen-Mediated Allergic Reaction in Rat Basophilic Leukemic RBL-2H3 Mast Cells and Passive Cutaneous Anaphylaxis in Mice
Figure 3
Effect of Rhus verniciflua extract (RVE) on the expression of FcεRI signaling pathway-related genes in RBL-2H3 mast cells. (a) Histamine levels in the culture medium of IgE-sensitized RBL-2H3 mast cells treated with RVE. (b, c) Immunoblot analysis performed with anti-p-Syk, -p-Lyn, -p-Fyn, -p-PLCγ1, -p-Akt, and -p-ERK antibodies. α-Tubulin was used as the protein loading control. Results are expressed as mean ± SE of at least five independent experimental results that were tested by analysis of variance with Bonferroni’s post hoc testing; , , and versus the control group; , , and versus the DNP-HSA-treated group.
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