Research Article
Development of a HPLC-MS/MS Method to Determine the 13 Elements of Semen Cuscutae and Application to a Pharmacokinetic Study in Rats
Table 1
The mass spectrometry of 13 compounds and IS.
| | Compound | Ion masses | Formula | Retention time | Precursor ion | Product ion | Fragment ions | CE (V) |
| | Caffeic acid | 180.157 | C9H8O4 | 6.57 | 179.1 | 135.0 | 135.0 | 12 | | Apigenin | 270.237 | C15H10O5 | 10.85 | 269.0 | 117.0 | 116.9, 151.2 | 34 | | Luteolin | 286.236 | C15H10O6 | 10.23 | 285.1 | 132.8 | 132.8 | 29 | | Kaempferol | 286.236 | C15H10O6 | 10.99 | 285.1 | 187.1 | 93.1, 117.2, 205.0 | 25 | | Quercetin | 302.236 | C15H10O7 | 10.33 | 300.9 | 151.0 | 197.8, 179.1, 151.0 | 15 | | Cryptochlorogenic acid | 354.309 | C16H18O9 | 5.33 | 353.1 | 173.2 | 191.0 | 11 | | Chlorogenic acid | 354.309 | C16H18O9 | 5.32 | 353.0 | 191.3 | 191.3 | 11 | | Quercitrin | 448.377 | C21H20O11 | 8.53 | 447.0 | 299.9 | 299.9 | 22 | | Astragalin | 448.377 | C21H20O11 | 8.22 | 477.1 | 284.1 | 284.1 | 25 | | Isoquercitrin | 464.376 | C21H20O12 | 6.68 | 462.9 | 300.0 | 300.0 | 24 | | Hyperoside | 464.376 | C21H20O12 | 6.73 | 463.1 | 300.0 | 300.0, 271.2 | 31 | | Isorhamnetin-3-O-glucoside | 478.403 | C22H22O12 | 8.36 | 476.9 | 313.8 | 300.9, 178.9, 150.9 | 10 | | Rutin | 610.518 | C27H30O16 | 5.81 | 609.2 | 300.1 | 300.1, 272.3 | 34 | | Liquiritin (IS) | 418.39 | C21H22O9 | 7.19 | 416.9 | 255.0 | 179.0 | 17 |
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