Research Article
Development of a HPLC-MS/MS Method to Determine the 13 Elements of Semen Cuscutae and Application to a Pharmacokinetic Study in Rats
Table 2
Calibration curves, correlation coefficients, linear ranges, and LLOQ of the analytes.
| | Compound | Calibration curves | Correlation coefficients (r) | Linear range (ng/mL) | LLOQ (ng/mL) |
| | Quercitrin | Y = 0.6491X + 0.0086 | 0.9925 | 1–250 | 1 | | Quercetin | Y = 0.5980X + 0.0026 | 0.9967 | 1–250 | 1 | | Hyperoside | Y = 0.8758X + 0.0098 | 0.9961 | 1–250 | 1 | | Caffeic acid | Y = 0.8239X + 0.0030 | 0.9986 | 1–250 | 1 | | Rutin | Y = 0.2459X + 0.0138 | 0.9920 | 2–500 | 2 | | Chlorogenic acid | Y = 0.1467X + 0.0038 | 0.9914 | 1–250 | 1 | | Luteolin | Y = 0.6678X + 0.0025 | 0.9936 | 1–250 | 1 | | Apigenin | Y = 0.7207X + 0.0017 | 0.9929 | 1–250 | 1 | | Kaempferol | Y = 0.0250X − 0.0001 | 0.9952 | 1–250 | 1 | | Isoquercitrin | Y = 0.8758X + 0.0098 | 0.9960 | 1–250 | 1 | | Cryptochlorogenic acid | Y = 0.1038X + 0.0003 | 0.9903 | 1–250 | 1 | | Isorhamnetin-3-O-glucoside | Y = 0.5362X + 0.0036 | 0.9942 | 1–250 | 1 | | Astragalin | Y = 0.6307X + 0.0052 | 0.9962 | 1–250 | 1 |
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