Subcutaneous Injection of Myrrh Essential Oil in Mice: Acute and Subacute Toxicity Study

Figure 2

Observation of physiological changes in skin after subcutaneous injection of MEO. (a) Mice were injected with sample containing 20 μL (A), 40 μL (B), and 80 μL (C) of MEO and 200 μL of hydrosol formulation (D) and normal saline (E), and the pictures were taken at the last day of experiment before sacrifice to evaluate any skin abnormalities. The MEO treated groups (A, B, and C) showed swelling (1) and some sort of wound (2) due to scratches by themselves. The F-200 (D) and normal saline (E) group did not show any kind of skin abnormalities. (b) Significant abrasions on the ears and neck region of mice in MEO (20, 40, and 80) groups.

(a)

(b)